| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Forcucci, Angela (14)
|
NITWST |
2:26.86 |
2:27.36 |
0.50 |
| 2 |
Harper, Amelia D (14)
|
NITWST |
|
2:28.88 |
- |
| 3 |
Drozdowski, Jessica L (13)
|
NITWST |
2:29.60 |
2:29.73 |
0.13 |
| 4 |
Clark, Greta N (13)
|
NITWST |
2:45.78 |
2:43.24 |
-2.54 |
| 5 |
Frerichs, Greta L (13)
|
NIHHSC |
2:47.37 |
2:47.13 |
-0.24 |
| 6 |
Fawls, Mia J (14)
|
NITWST |
|
2:49.01 |
- |
| 7 |
Astalos, Holly T (14)
|
NIHAMB |
2:56.76 |
2:53.32 |
-3.44 |
| 8 |
Keesler, Kaylynn N (14)
|
NIEMAC |
2:51.92 |
2:57.19 |
5.27 |
| 9 |
Judy, Paris M (14)
|
NITWST |
3:04.60 |
2:57.29 |
-7.31 |
| 10 |
Mancuso, Gabriella J (13)
|
NIHAMB |
3:18.29 |
3:02.32 |
-15.97 |
| 11 |
Hardy, Haleigha G (13)
|
NIPVSC |
3:18.14 |
3:08.90 |
-9.24 |
| 12 |
Barry, Caitlin E (14)
|
NITWST |
3:07.15 |
3:10.56 |
3.41 |
| 13 |
Regan, Maggie E (13)
|
NITWST |
3:35.33 |
3:24.69 |
-10.64 |
| 14 |
Hellwig, Ashleigh R (14)
|
NIHHSC |
3:36.77 |
3:36.52 |
-0.25 |
|
Jones, Sophia M (13)
|
NIPVSC |
3:13.42 |
DQ |
- |